Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Immunohistochemical analysis of F4/80 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. B F4/80 scores in each group (n = 5/group). C Immunohistochemical analysis of CD86 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. D Immunohistochemical analysis of CD163 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. ( E ) CD86/CD163 expression ratio (M1/M2 ratio) in each group (n = 5/group). SVF, stromal vascular fraction; ADSC, adipose-derived stromal cell; DMM, destabilization of the medial meniscus; MΦ, macrophages

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Immunohistochemical staining, Expressing, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Immunofluorescence analysis of F4/80, CD163, and hNA in synovial tissue of the rats at 4 weeks after DMM showing representative triple immunostaining of F4/80 (green), CD163 (red), and hNA (violet) in each group. Scale bar = 20 μm. B Comparison of the ratio of CD163- and hNA-positive cells in the F4/80-positive cells via immunofluorescence staining (n = 5/group). SVF, stromal vascular fraction; ADSC, adipose-derived stromal cell; hNA, human nuclear antigen

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Immunofluorescence, Triple Immunostaining, Comparison, Staining, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Flow cytometry analysis revealing the proportion of CD163-positive cells in the SVF (n = 5). B Setup of the separated pellet co-culture system. Groups including ADSC, M2Φ, and SVF were established, with each administered cell type in membrane plates and OA chondrocytes in 15 mL tubes. A control group was established with no cells in membrane plates and only OA chondrocytes in 15-mL tubes. C Gross photographs and safranin-O staining of the resulting pellets in each group. D Comparison of pellet size among each group (n = 5/group). E Analysis of TGF-β, IL-10, and MMP-13 levels in the supernatant following coculture with ADSC, M2Φ, and SVF and the chondrocyte (n = 5/group). SVF, stromal vascular fraction; M2Φ, M2 macrophages; ADSC, adipose-derived stromal cell; TGF-β, transforming growth factor-β; IL-10, interleukin-10; MMP-13, matrix metalloproteinase 13

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Flow Cytometry, Co-Culture Assay, Membrane, Control, Staining, Comparison, Derivative Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

doi: 10.1155/2020/2946820

Figure Lengend Snippet: Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF- α , and (b) M2 polarization-related genes, including ARG, IL10, TGF- β , CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. ( ∗ p < 0.05; † p < 0.01). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.

Article Snippet: LPS (L4391, Sigma) was used with a concentration of 10 ng/mL, SNAP (S-Nitroso-N-Acetyl-D,L-Penicillamine; Sigmal) was used with a concentration of 50 μ M, and cPTIO (2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide, monopotassium salt; Cayman) was used with a concentration of 50 μ M. For staining with antibodies, anti-iNOS-PE (12-5920-80, eBioscience), anti-CD163-PE (bs-2527R-PE, Bioss), and anti-CD86-FITC (bs-1035R-FITC, Bioss) were used.

Techniques: Flow Cytometry

Journal: Brain, behavior, and immunity

Article Title: Intermittent cytomegalovirus infection alters neurobiological metabolism and induces cognitive deficits in mice

doi: 10.1016/j.bbi.2023.12.033

Figure Lengend Snippet: Brain cell characterization flow panel.

Article Snippet: CD163 , AF680 , Bioss , bs-2527R-A680 , 1:400.

Techniques: